Specialty PCR Enzymes

Specialty PCR    l 2013 PCR Enzyme and Mastermix Catalog l
ACTaq™ High Fidelity DNA Polymerase
ACTaq™ Blue High Fidelity DNA Polymerase
ACTaq™ Long DNA Polymerase
ACTaq™ Blue Long DNA Polymerase
ACTaq™ High Fidelity DNA Polymerase  P/N: E2000
Product Description:           ACTaq™ High Fidelity  DNA Polymerase,
originating from Pyrococcus furiosus with molecular weight of 90KDa,
 is a thermo stable enzyme and exhibits the lower error rate of DNA amplification.
 For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease
proofreading activity; at extension amplification speed of 1000 bases per minute
 and optimized between 65-75oC, when using dNTP concentration at
100-300μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1.
 ACTaq™ High Fidelity DNA Polymerase generates blunt-ended fragment.
Storage Condition:                  -20oC. Stable for at least 12 months in
constant freezer temperature.
Concentration:                          5 Units/μL
Unit Definition:                       One unit of the amount of enzyme that incorporates
10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.
Product Components:        ACTaq™ High Fidelity DNA Polymerase, 10x PCR reaction
 buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA,
 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol.
PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4, 100mM KCl,
160mM
(NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
Quality Control:                      Activity of PCR for 1Kb fragment.  Free of contaminating
 endonucleases, exonucleases, and nicking activities.  The purity of the enzyme is
 evaluated by SDS-PAGE at >95% purity.
ACTaq™ Blue High Fidelity DNA Polymerase
P/N: E2000B (blue)
Product Description:           ACTaq™ High Fidelity  DNA Polymerase, originating
from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable
enzyme and exhibits the lower error rate of DNA amplification.  For use in DNA
fragment extension amplification reactions with 3' to 5' exonuclease  proofreading
activity; at extension amplification speed of 1000 bases per minute and optimized
between 65-75oC, when using dNTP concentration at 100-300μM, Mg2+ concentration
at 2.0-3.0mM, and pH at 8.1-9.1.  ACTaq™ High Fidelity DNA Polymerase generates
 blunt-ended fragment.
Storage Condition:                  -20oC. Stable for at least 12 months in constant freezer
temperature.
Concentration:                            1 Unit/μL
Unit Definition:                       One unit of the amount of enzyme that incorporates
10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.
Product Components:        ACTaq™ Blue High Fidelity DNA Polymerase,
10x PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM
EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol.
PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4,
100mM KCl, 160mM
(NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
Quality Control:                      Activity of PCR for 1Kb fragment.  Free of
contaminating endonucleases, exonucleases, and nicking activities.  The purity
 of the enzyme is evaluated by SDS-PAGE at >95% purity.
ACTaq™ Long DNA Polymerase
Product Description:           ACTaq™ Long DNA Polymerase is a specialty taq
 for high processivity and fidelity at extension amplification speed fo 1000 bases per
 minute and optimized b etween 65-75oC, when using dNTP concentration at
100-500μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.2.
Storage Condition:                  -20oC. Stable for at least 12 months in constant
freezer temperature.
Concentration:                          5 Units/μL
Unit Definition:                       One unit of the amount of enzyme that incorporates
10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.
Product Components:        ACTaq™ Long DNA Polymerase, 10x PCR reaction
buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl,
0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol.
PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4,
100mM KCl, 160mM
(NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
Quality Control:                      ACTaq™ Long DNA Polymerase is highly
 purified.  Free of contaminating endonucleases, exonucleases, and nicking
 activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.
ACTaq™ Blue Long DNA Polymerase
Product Description:           ACTaq™ Long DNA Polymerase is a specialty
 taq for high processivity and fidelity at extension amplification speed fo 1000
bases per minute and optimized b etween 65-75oC, when using dNTP
concentration at 100-500μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.2.
Storage Condition:                  -20oC. Stable for at least 12 months in
constant freezer temperature.
Concentration:                            1 Unit/μL
Unit Definition:                       One unit of the amount of enzyme that incorporates
 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.
Product Components:        ACTaq™ Blue Long DNA Polymerase, 10x
PCR reaction buffer with Mg2+
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer:                       10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM
 EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol.
PCR Reaction Buffer:            10x PCR buffer with Mg2+.  20mM MgSO4,
100mM KCl, 160mM
(NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
Quality Control:                      ACTaq™ Long DNA Polymerase is highly
 purified.  Free of contaminating endonucleases, exonucleases, and nicking
activities.  The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.

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