Standard PCR l 2013 PCR Enzyme and Mastermix Catalog l
Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus
with molecular
weight of 94KDa. For use in DNA fragment extension amplification
reactions with DNA having dA overhang on 3’ ends; at extension
amplification speed of 1200 bases per minute and optimized between
65-75oC. Optimized for best performance when using dNTP concentration
at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.
weight of 94KDa. For use in DNA fragment extension amplification
reactions with DNA having dA overhang on 3’ ends; at extension
amplification speed of 1200 bases per minute and optimized between
65-75oC. Optimized for best performance when using dNTP concentration
at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.
Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.
Concentration: 5 Units/μL
Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.
acid-insoluble material in 30 minutes at 72oC.
Product Components: ACTaq™ DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2.
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.
PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
ACTaq™ Blue DNA Polymerase
ACTaq™ Red DNA Polymerase
P/N:E2100B (blue), E2100R (red)
Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with
P/N:E2100B (blue), E2100R (red)
Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with
molecular
weight of 94KDa. ACTaq™ Blue or Red differs from ACTaq™ DNA Polymerase in that
weight of 94KDa. ACTaq™ Blue or Red differs from ACTaq™ DNA Polymerase in that
it contains blue / red dye added to the enzyme used as tracking and loading dye as no
loading buffer is required.
Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.
Concentration: 1 Unit/μL
Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.
acid-insoluble material in 30 minutes at 72oC.
Product Components: ACTaq™ Blue or Red DNA Polymerase, PCR reaction buffer,
10X 25mM MgCl2.
(Buffer and MgCl2 concentrations are customizable upon request)
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.
PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
Quality Control: ACTaq™ Blue or Red DNA Polymerase is highly purified. Free of
contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
PCR test results for ACTaq™ DNA Poly,
ACTaq™ Blue DNA Poly,
ACTaq™ Hot-Start DNA Poly,
and ACTaq™ Mastermix
1 DNA Marker
2 ACTaq™ DNA Poly
3 ACTaq™ Blue DNA Poly
4 ACTaq™ Hot-Start DNA Poly
5 ACTaq™ Mastermix
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